Biochemical properties of gastrokine-1 purified from chicken gizzard smooth muscle.

ABSTRACT:

Unlabelled

The potential role and function of gastrokine-1 (GNK1) in smooth muscle cells is investigated in this work by first establishing a preparative protocol to obtain this native protein from freshly dissected chicken gizzard. Some unexpected biochemical properties of gastrokine-1 were deduced by producing specific polyclonal antibody against the purified protein. We focused on the F-actin interaction with gastrokine-1 and the potential role and function in smooth muscle contractile properties.

Background

GNK1 is thought to provide mucosal protection in the superficial gastric epithelium. However, the actual role of gastrokine-1 with regards to its known decreased expression in gastric cancer is still unknown. Recently, trefoil factors (TFF) were reported to have impor

SUBMITTER: Hnia K 

PROVIDER: S-EPMC2588339 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature