Combinatorial chemoenzymatic synthesis and high-throughput screening of sialosides.

ABSTRACT: Although the vital roles of structures containing sialic acid in biomolecular recognition are well documented, limited information is available on how sialic acid structural modifications, sialyl linkages, and the underlying glycan structures affect the binding or the activity of sialic acid-recognizing proteins and related downstream biological processes. A novel combinatorial chemoenzymatic method has been developed for the highly efficient synthesis of biotinylated sialosides containing different sialic acid structures and different underlying glycans in 96-well plates from biotinylated sialyltransferase acceptors and sialic acid precursors. By transferring the reaction mixtures to NeutrAvidin-coated plates and assaying for the yields of enzymatic reactions using lectins recognizing sia

SUBMITTER: Chokhawala HA 

PROVIDER: S-EPMC2588434 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature