Genotypic and phenotypic diversity of Bacillus spp. isolated from steel plant waste.

ABSTRACT:

Background

Molecular studies of Bacillus diversity in various environments have been reported. However, there have been few investigations concerning Bacillus in steel plant environments. In this study, genotypic and phenotypic diversity and phylogenetic relationships among 40 bacterial isolates recovered from steel plant waste were investigated using classical and molecular methods.

Results

16S rDNA partial sequencing assigned all the isolates to the Bacillus genus, with close genetic relatedness to the Bacillus subtilis and Bacillus cereus groups, and to the species Bacillus sphaericus. tDNA-intergenic spacer length polymorphisms and the 16S-23S intergenic transcribed spacer region failed to identify the isolates at the species level. Genomic diversity was investigated by

SUBMITTER: Freitas DB 

PROVIDER: S-EPMC2588453 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature