Exploring valid reference genes for gene expression studies in Brachypodium distachyon by real-time PCR.

ABSTRACT:

Background

The wild grass species Brachypodium distachyon (Brachypodium hereafter) is emerging as a new model system for grass crop genomics research and biofuel grass biology. A draft nuclear genome sequence is expected to be publicly available in the near future; an explosion of gene expression studies will undoubtedly follow. Therefore, stable reference genes are necessary to normalize the gene expression data.

Results

A systematic exploration of suitable reference genes in Brachypodium is presented here. Nine reference gene candidates were chosen, and their gene sequences were obtained from the Brachypodium expressed sequence tag (EST) databases. Their expression levels were examined by quantitative real-time PCR (qRT-PCR) using 21 different Brachypodium plant samples, i

SUBMITTER: Hong SY 

PROVIDER: S-EPMC2588586 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature