PCR and real-time PCR primers developed for detection and identification of Bifidobacterium thermophilum in faeces.

ABSTRACT:

Background

Culture-independent methods based on the 16S ribosomal RNA molecule are nowadays widely used for assessment of the composition of the intestinal microbiota, in relation to host health or probiotic efficacy. Because Bifidobacterium thermophilum was only recently isolated from human faeces until now, no specific real-time PCR (qPCR) assay has been developed for detection of this species as component of the bifidobacterial community of the human intestinal flora.

Results

Design of specific primers and probe was achieved based on comparison of 108 published bifidobacterial 16S rDNA sequences with the recently published sequence of the human faecal isolate B. thermophilum RBL67. Specificity of the primer was tested in silico by similarity search against the sequence da

SUBMITTER: Mathys S 

PROVIDER: S-EPMC2588598 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature