The cotranslational maturation program for the type II membrane glycoprotein influenza neuraminidase.

ABSTRACT: The earliest steps in nascent protein maturation greatly affect its overall efficiency. Constraints placed on maturing proteins at these early stages limit available conformations and help to direct the native maturation process. For type II membrane proteins, these cotranslational constraints include N- and C-terminal membrane tethering, chaperone binding, and disulfide bond formation. The cotranslational maturation process for the type II membrane glycoprotein influenza neuraminidase (NA) was investigated to provide a deeper understanding of these initial endoplasmic reticulum events. The type II orientation provides experimental advantages to monitor the first maturation steps. Calnexin was shown to cotranslationally interact with NA prior to calreticulin. These interactions were requir

SUBMITTER: Wang N 

PROVIDER: S-EPMC2590707 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature