Conformational analysis of a covalently cross-linked Watson-Crick base pair model.

ABSTRACT: Low-temperature NMR experiments and molecular modeling have been used to characterize the conformational behavior of a covalently cross-linked DNA base pair model. The data suggest that Watson-Crick or reverse Watson-Crick hydrogen bonding geometries have similar energies and can interconvert at low temperatures. This low-temperature process involves rotation about the crosslink CH(2)C(5') (psi) carbon-carbon bond, which is energetically preferred over the alternate CH(2)N(3) (phi) carbon-nitrogen bond rotation.

SUBMITTER: Jensen EA 

PROVIDER: S-EPMC2590868 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature