Ontology highlight
ABSTRACT:
SUBMITTER: Nogi T
PROVIDER: S-EPMC2590912 | biostudies-literature | 2008 Dec
REPOSITORIES: biostudies-literature
Protein science : a publication of the Protein Society 20080911 12
Biologically important human proteins often require mammalian cell expression for structural studies, presenting technical and economical problems in the production/purification processes. We introduce a novel affinity peptide tagging system that uses a low affinity anti-peptide monoclonal antibody. Concatenation of the short recognition sequence enabled the successful engineering of an 18-residue affinity tag with ideal solution binding kinetics, providing a low-cost purification means when com ...[more]