The Kv2.1 K+ channel targets to the axon initial segment of hippocampal and cortical neurons in culture and in situ.

ABSTRACT:

Background

The Kv2.1 delayed-rectifier K+ channel regulates membrane excitability in hippocampal neurons where it targets to dynamic cell surface clusters on the soma and proximal dendrites. In the past, Kv2.1 has been assumed to be absent from the axon initial segment.

Results

Transfected and endogenous Kv2.1 is now demonstrated to preferentially accumulate within the axon initial segment (AIS) over other neurite processes; 87% of 14 DIV hippocampal neurons show endogenous channel concentrated at the AIS relative to the soma and proximal dendrites. In contrast to the localization observed in pyramidal cells, GAD positive inhibitory neurons within the hippocampal cultures did not show AIS targeting. Photoactivable-GFP-Kv2.1-containing clusters at the AIS were stable, moving

SUBMITTER: Sarmiere PD 

PROVIDER: S-EPMC2592246 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature