Microbial biofilm voltammetry: direct electrochemical characterization of catalytic electrode-attached biofilms.

ABSTRACT: While electrochemical characterization of enzymes immobilized on electrodes has become common, there is still a need for reliable quantitative methods for study of electron transfer between living cells and conductive surfaces. This work describes growth of thin (<20 microm) Geobacter sulfurreducens biofilms on polished glassy carbon electrodes, using stirred three-electrode anaerobic bioreactors controlled by potentiostats and nondestructive voltammetry techniques for characterization of viable biofilms. Routine in vivo analysis of electron transfer between bacterial cells and electrodes was performed, providing insight into the main redox-active species participating in electron transfer to electrodes. At low scan rates, cyclic voltammetry revealed catalytic electron transfer between cel

SUBMITTER: Marsili E 

PROVIDER: S-EPMC2592900 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature