PBAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria.

ABSTRACT: We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the P(BAD) promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.

SUBMITTER: Qiu D 

PROVIDER: S-EPMC2592904 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature