Differential expression in Phanerochaete chrysosporium of membrane-associated proteins relevant to lignin degradation.

ABSTRACT: Fungal lignin-degrading systems likely include membrane-associated proteins that participate in diverse processes such as uptake and oxidation of lignin fragments, production of ligninolytic secondary metabolites, and defense of the mycelium against ligninolytic oxidants. Little is known about the nature or regulation of these membrane-associated components. We grew the white rot basidiomycete Phanerochaete chrysosporium on cellulose or glucose as the carbon source and monitored the mineralization of a (14)C-labeled synthetic lignin by these cultures to assess their ligninolytic competence. The results showed that the cellulose-grown cultures were ligninolytic, whereas the glucose-grown ones were not. We isolated microsomal membrane fractions from both types of culture and analyzed tryptic

SUBMITTER: Shary S 

PROVIDER: S-EPMC2592923 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature