YKu70/yKu80 and Rif1 regulate silencing differentially at telomeres in Candida glabrata.

ABSTRACT: Candida glabrata, a common opportunistic fungal pathogen, adheres efficiently to mammalian epithelial cells in culture. This interaction in vitro depends mainly on the adhesin Epa1, one of a large family of cell wall proteins. Most of the EPA genes are located in subtelomeric regions, where they are transcriptionally repressed by silencing. In order to better characterize the transcriptional regulation of the EPA family, we have assessed the importance of C. glabrata orthologues of known regulators of subtelomeric silencing in Saccharomyces cerevisiae. To this end, we used a series of strains containing insertions of the reporter URA3 gene within different intergenic regions throughout four telomeres of C. glabrata. Using these reporter strains, we have assessed the roles of SIR2, SIR3, SI

SUBMITTER: Rosas-Hernandez LL 

PROVIDER: S-EPMC2593182 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature