Posttranscriptional regulation of flagellin synthesis in Helicobacter pylori by the RpoN chaperone HP0958.

ABSTRACT: The Helicobacter pylori protein HP0958 is essential for flagellum biogenesis. It has been shown that HP0958 stabilizes the sigma(54) factor RpoN. The aim of this study was to further investigate the role of HP0958 in flagellum production in H. pylori. Global transcript analysis identified a number of flagellar genes that were differentially expressed in an HP0958 mutant strain. Among these, the transcription of the major flagellin gene flaA was upregulated twofold, suggesting that HP0958 was a negative regulator of the flaA gene. However, the production of the FlaA protein was significantly reduced in the HP0958 mutant, and this was not due to the decreased stability of the FlaA protein. RNA stability analysis and binding assays indicated that HP0958 binds and destabilizes flaA mRNA. The H

SUBMITTER: Douillard FP 

PROVIDER: S-EPMC2593243 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature