The JmjC domain histone demethylase Ndy1 regulates redox homeostasis and protects cells from oxidative stress.

ABSTRACT: The histone H3 demethylase Ndy1/KDM2B protects cells from replicative senescence. Changes in the metabolism of reactive oxygen species (ROS) are important for establishing senescence, suggesting that Ndy1 may play a role in redox regulation. Here we show that Ndy1 protects from H(2)O(2)-induced apoptosis and G(2)/M arrest and inhibits ROS-mediated signaling and DNA damage, while knockdown of Ndy1 has the opposite effects. Consistent with these observations, whereas Ndy1 overexpression promotes H(2)O(2) detoxification, Ndy1 knockdown inhibits it. Ndy1 promotes the expression of genes encoding the antioxidant enzymes aminoadipic semialdehyde synthase (Aass), NAD(P)H quinone oxidoreductase-1 (Nqo1), peroxiredoxin-4 (Prdx4), and serine peptidase inhibitor b1b (Serpinb1b) and represses the expr

SUBMITTER: Polytarchou C 

PROVIDER: S-EPMC2593427 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature