CrossHybDetector: detection of cross-hybridization events in DNA microarray experiments.

ABSTRACT:

Background

DNA microarrays contain thousands of different probe sequences represented on their surface. These are designed in such a way that potential cross-hybridization reactions with non-target sequences are minimized. However, given the large number of probes, the occurrence of cross hybridization events cannot be excluded. This problem can dramatically affect the data quality and cause false positive/false negative results.

Results

CrossHybDetector is a software package aimed at the identification of cross-hybridization events occurred during individual array hybridization, by using the probe sequences and the array intensity values. As output, the software provides the user with a list of array spots potentially 'corrupted' and their associated p-values calculated by

SUBMITTER: Uva P 

PROVIDER: S-EPMC2596149 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature