Cytokine response after severe respiratory syncytial virus bronchiolitis in early life.

ABSTRACT:

Background

Immune response after viral infection usually involves T(H)1-mediated response; however, severe respiratory syncytial virus (RSV) infection appears to be associated with the development of asthma, a T(H)2-predominant phenotype.

Objective

To understand the early and subsequent immunologic response to a serious RSV infection in children over time.

Methods

A total of 206 previously healthy infants hospitalized with severe RSV bronchiolitis were enrolled in a prospective cohort called the RSV Bronchiolitis in Early Life study. Peripheral blood T cells were obtained immediately after RSV infection and at 2, 4, and 6 years of age, stimulated with phorbol 12-myristate 13-acetate and ionomycin, and analyzed for IL-2, IL-4, IL-13, and IFN-gamma by flow cytometry and

SUBMITTER: Castro M 

PROVIDER: S-EPMC2597800 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature