Ultra-high resolution array painting facilitates breakpoint sequencing.

ABSTRACT:

Objective

To describe a considerably advanced method of array painting, which allows the rapid, ultra-high resolution mapping of translocation breakpoints such that rearrangement junction fragments can be amplified directly and sequenced.

Method

Ultra-high resolution array painting involves the hybridisation of probes generated by the amplification of small numbers of flow-sorted derivative chromosomes to oligonucleotide arrays designed to tile breakpoint regions at extremely high resolution.

Results and discussion

How ultra-high resolution array painting of four balanced translocation cases rapidly and efficiently maps breakpoints to a point where junction fragments can be amplified easily and sequenced is demonstrated. With this new development, breakpoints can be m

SUBMITTER: Gribble SM 

PROVIDER: S-EPMC2597908 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature