Toll-like receptor signaling in small intestinal epithelium promotes B-cell recruitment and IgA production in lamina propria.

ABSTRACT:

Background & aims

Several lines of evidence support a role for Toll-like receptor (TLR) signaling to protect the intestine from pathogenic infection. We hypothesized that TLR signaling at the level of the intestinal epithelium is critical for mucosal immune responses.

Methods

We generated transgenic mice that express a constitutively active form of TLR4 in the intestinal epithelium (V-TLR4 mice). Lamina propria cellularity was evaluated by immunostaining and flow cytometry. Immunoglobulin (Ig) A levels in the stool and serum were measured by enzyme-linked immunosorbent assay. Chemokine and cytokine expression were analyzed by quantitative polymerase chain reaction and enzyme-linked immunosorbent assay.

Results

V-TLR4 transgenic mice reproduced normally and had a norma

SUBMITTER: Shang L 

PROVIDER: S-EPMC2598776 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature