Identification of G1-regulated genes in normally cycling human cells.

ABSTRACT:

Background

Obtaining synchronous cell populations is essential for cell-cycle studies. Methods such as serum withdrawal or use of drugs which block cells at specific points in the cell cycle alter cellular events upon re-entry into the cell cycle. Regulatory events occurring in early G1 phase of a new cell cycle could have been overlooked.

Methodology and findings

We used a robotic mitotic shake-off apparatus to select cells in late mitosis for genome-wide gene expression studies. Two separate microarray experiments were conducted, one which involved isolation of RNA hourly for several hours from synchronous cell populations, and one experiment which examined gene activity every 15 minutes from late telophase of mitosis into G1 phase. To verify synchrony of the cell populati

SUBMITTER: Beyrouthy MJ 

PROVIDER: S-EPMC2600614 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature