The temporal program of chromosome replication: genomewide replication in clb5{Delta} Saccharomyces cerevisiae.

ABSTRACT: Temporal regulation of origin activation is widely thought to explain the pattern of early- and late-replicating domains in the Saccharomyces cerevisiae genome. Recently, single-molecule analysis of replication suggested that stochastic processes acting on origins with different probabilities of activation could generate the observed kinetics of replication without requiring an underlying temporal order. To distinguish between these possibilities, we examined a clb5Delta strain, where origin firing is largely limited to the first half of S phase, to ask whether all origins nonspecifically show decreased firing (as expected for disordered firing) or if only some origins ("late" origins) are affected. Approximately half the origins in the mutant genome show delayed replication while the rema

SUBMITTER: McCune HJ 

PROVIDER: S-EPMC2600925 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature