Homologous recombination-mediated cloning and manipulation of genomic DNA regions using Gateway and recombineering systems.

ABSTRACT:

Background

Employing genomic DNA clones to characterise gene attributes has several advantages over the use of cDNA clones, including the presence of native transcription and translation regulatory sequences as well as a representation of the complete repertoire of potential splice variants encoded by the gene. However, working with genomic DNA clones has traditionally been tedious due to their large size relative to cDNA clones and the presence, absence or position of particular restriction enzyme sites that may complicate conventional in vitro cloning procedures.

Results

To enable efficient cloning and manipulation of genomic DNA fragments for the purposes of gene expression and reporter-gene studies we have combined aspects of the Gateway system and a bacteriophage-based

SUBMITTER: Rozwadowski K 

PROVIDER: S-EPMC2601046 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature