Rapid control of protein level in the apicomplexan Toxoplasma gondii.
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ABSTRACT:
SUBMITTER: Herm-Gotz A
PROVIDER: S-EPMC2601725 | biostudies-literature | 2007 Dec
REPOSITORIES: biostudies-literature
Publications
Rapid control of protein level in the apicomplexan Toxoplasma gondii.
Herm-Götz Angelika A Agop-Nersesian Carolina C Münter Sylvia S Grimley Joshua S JS Wandless Thomas J TJ Frischknecht Friedrich F Meissner Markus M
Nature methods 20071111 12
Analysis of gene function in apicomplexan parasites is limited by the absence of reverse genetic tools that allow easy and rapid modulation of protein levels. The fusion of a ligand-controlled destabilization domain (ddFKBP) to a protein of interest enables rapid and reversible protein stabilization in T. gondii. This allows an efficient functional analysis of proteins that have a dual role during host cell invasion and/or intracellular growth of the parasite. ...[more]
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