Regulation of hematopoiesis by the BMP signaling pathway in adult zebrafish.

ABSTRACT:

Objective

The zebrafish is an established model system for studying the embryonic emergence of tissues and organs, including the hematopoietic system. We hypothesized that key signaling pathways controlling embryonic hematopoiesis continue to be important in the adult, and we sought to develop approaches to test this in zebrafish, focused on the bone morphogenetic protein (BMP) signaling pathway. Functions for this pathway in adult hematopoiesis have been challenging to probe in other models.

Materials and methods

Several approaches tested the function of BMP signaling during adult zebrafish hematopoiesis. First, we evaluated steady-state hematopoiesis in adult fish that are heterozygous for mutant alleles of Smad5, or are homozygous for mutant alleles, and rescued to adulth

SUBMITTER: McReynolds LJ 

PROVIDER: S-EPMC2602881 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature