Integrated Bioinformatics for Radiation-Induced Pathway Analysis from Proteomics and Microarray Data.

ABSTRACT: Functional analysis and interpretation of large-scale proteomics and gene expression data require effective use of bioinformatics tools and public knowledge resources coupled with expert-guided examination. An integrated bioinformatics approach was used to analyze cellular pathways in response to ionizing radiation. ATM, or ataxia-telangiectasia mutated , a serine-threonine protein kinase, plays critical roles in radiation responses, including cell cycle arrest and DNA repair. We analyzed radiation responsive pathways based on 2D-gel/MS proteomics and microarray gene expression data from fibroblasts expressing wild type or mutant ATM gene. The analysis showed that metabolism was significantly affected by radiation in an ATM dependent manner. In particular, purine metabolic pathways were di

SUBMITTER: Hu ZZ 

PROVIDER: S-EPMC2603135 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature