SERPINA3K prevents oxidative stress induced necrotic cell death by inhibiting calcium overload.

ABSTRACT:

Background

SERPINA3K, an extracellular serine proteinase inhibitor (serpin), has been shown to have decreased levels in the retinas of diabetic rats, which may contribute to diabetic retinopathy. The function of SERPINA3K in the retina has not been investigated.

Methodology/principal findings

The present study identified a novel function of SERPINA3K, i.e. it protects retinal cells against oxidative stress-induced cell death including retinal neuronal cells and Müller cells. Flow-cytometry showed that the protective effect of SERPINA3K on Müller cells is via reducing oxidation-induced necrosis. Measurements of intracellular calcium concentration showed that SERPINA3K prevented the intracellular calcium overload induced by H(2)O(2). A similar protective effect was observed us

SUBMITTER: Zhang B 

PROVIDER: S-EPMC2605247 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature