Microarray analysis of Foxa2 mutant mouse embryos reveals novel gene expression and inductive roles for the gastrula organizer and its derivatives.

ABSTRACT:

Background

The Spemann/Mangold organizer is a transient tissue critical for patterning the gastrula stage vertebrate embryo and formation of the three germ layers. Despite its important role during development, there are still relatively few genes with specific expression in the organizer and its derivatives. Foxa2 is a forkhead transcription factor that is absolutely required for formation of the mammalian equivalent of the organizer, the node, the axial mesoderm and the definitive endoderm (DE). However, the targets of Foxa2 during embryogenesis, and the molecular impact of organizer loss on the gastrula embryo, have not been well defined.

Results

To identify genes specific to the Spemann/Mangold organizer, we performed a microarray-based screen that compared wild-type and

SUBMITTER: Tamplin OJ 

PROVIDER: S-EPMC2605479 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature