Sphingosine-1-phosphate receptor subtypes differentially regulate smooth muscle cell phenotype.

ABSTRACT:

Objective

The role of sphingosine-1-phosphate (S1P) receptors in acute vascular injury and smooth muscle cell (SMC) phenotypic modulation is not completely resolved.

Methods and results

S1P receptor antagonists were used to test the hypothesis that specific S1P receptor subtypes differentially regulate SMC phenotypic modulation. In response to acute balloon injury of the rat carotid artery, S1P1/S1P3 receptor mRNA levels were transiently increased at 48 hours whereas S1P2 receptor expression was decreased. S1P2 expression was reinduced and increased at 7 to 10 days postinjury. Daily intraperitoneal injection of the S1P1/S1P3 antagonist VPC44116 decreased neointimal hyperplasia by approximately 50%. In vitro, pharmacological inhibition of S1P1/S1P3 receptors with VPC25239 att

SUBMITTER: Wamhoff BR 

PROVIDER: S-EPMC2605659 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature