Confirmation of gene expression-based prediction of survival in non-small cell lung cancer.

ABSTRACT:

Purpose

It is a critical challenge to determine the risk of recurrence in early stage non-small cell lung cancer (NSCLC) patients. Accurate gene expression signatures are needed to classify patients into high- and low-risk groups to improve the selection of patients for adjuvant therapy.

Experimental design

Multiple published microarray data sets were used to evaluate our previously identified lung cancer prognostic gene signature. Expression of the signature genes was further validated with real-time reverse transcription-PCR and Western blot assays of snap-frozen lung cancer tumor tissues.

Results

Our previously identified 35-gene signature stratified 264 patients with NSCLC into high- and low-risk groups with distinct overall survival rates (P < 0.05, Kaplan-Meier

SUBMITTER: Guo NL 

PROVIDER: S-EPMC2605664 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature