Expressed sequence tag analysis of guinea pig (Cavia porcellus) eye tissues for NEIBank.

ABSTRACT:

Purpose

To characterize gene expression patterns in guinea pig ocular tissues and identify orthologs of human genes from NEIBank expressed sequence tags.

Methods

RNA was extracted from dissected eye tissues of 2.5-month-old guinea pigs to make three unamplified and unnormalized cDNA libraries in the pCMVSport-6 vector for the lens, retina, and eye minus lens and retina. Over 4,000 clones were sequenced from each library and were analyzed using GRIST for clustering and gene identification. Lens crystallin EST data were validated using two-dimensional electrophoresis (2-DE), matrix assisted laser desorption (MALDI), and electrospray ionization mass spectrometry (ESIMS).

Results

Combined data from the three libraries generated a total of 6,694 distinctive gene clusters,

SUBMITTER: Simpanya MF 

PROVIDER: S-EPMC2605723 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature