Cross-kingdom amplification using bacteria-specific primers: complications for studies of coral microbial ecology.

ABSTRACT: PCR amplification of pure bacterial DNA is vital to the study of bacterial interactions with corals. Commonly used Bacteria-specific primers 8F and 27F paired with the universal primer 1492R amplify both eukaryotic and prokaryotic rRNA genes. An alternative primer set, 63F/1542R, is suggested to resolve this problem.

SUBMITTER: Galkiewicz JP 

PROVIDER: S-EPMC2607152 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature