Resequencing microarray probe design for typing genetically diverse viruses: human rhinoviruses and enteroviruses.

ABSTRACT:

Background

Febrile respiratory illness (FRI) has a high impact on public health and global economics and poses a difficult challenge for differential diagnosis. A particular issue is the detection of genetically diverse pathogens, i.e. human rhinoviruses (HRV) and enteroviruses (HEV) which are frequent causes of FRI. Resequencing Pathogen Microarray technology has demonstrated potential for differential diagnosis of several respiratory pathogens simultaneously, but a high confidence design method to select probes for genetically diverse viruses is lacking.

Results

Using HRV and HEV as test cases, we assess a general design strategy for detecting and serotyping genetically diverse viruses. A minimal number of probe sequences (26 for HRV and 13 for HEV), which were potentially

SUBMITTER: Wang Z 

PROVIDER: S-EPMC2607299 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature