A toolbox for epitope-tagging and genome-wide location analysis in Candida albicans.

ABSTRACT:

Background

Candida albicans is a diploid pathogenic fungus not yet amenable to routine genetic investigations. Understanding aspects of the regulation of its biological functions and the assembly of its protein complexes would lead to further insight into the biology of this common disease-causing microbial agent.

Results

We have developed a toolbox allowing in vivo protein tagging by PCR-mediated homologous recombination with TAP, HA and MYC tags. The transformation cassettes were designed to accommodate a common set of integration primers. The tagged proteins can be used to perform tandem affinity purification (TAP) or chromatin immunoprecipitation coupled with microarray analysis (ChIP-CHIP). Tandem affinity purification of C. albicans Nop1 revealed the high conservation

SUBMITTER: Lavoie H 

PROVIDER: S-EPMC2607300 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature