Single nucleotide polymorphism-based system improves the applicability of quantitative PCR for chimerism monitoring.

ABSTRACT: Recently, several studies demonstrated the feasibility of a real-time quantitative PCR (qPCR) approach for chimerism monitoring. qPCR offers a fast, sensitive, and elegant quantification of genotypes. However, before it becomes an established method for routine chimerism monitoring, a qPCR marker set for every transplant pair should be available. This requirement poses a major challenge since the genetic markers for qPCR--short insertions/deletions (Indels) and single nucleotide polymorphisms (SNPs)--published to-date do not guarantee applicability for every transplant pair. The aim of our study was to design and validate a new SNP allele-specific system to supplement an already existing Indel primer panel and improve applicability of the qPCR approach for chimerism status monitoring. Here

SUBMITTER: Gineikiene E 

PROVIDER: S-EPMC2607568 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature