Gene expression profile of cervical and skin tissues from human papillomavirus type 16 E6 transgenic mice.

ABSTRACT:

Background

Although K14E6 transgenic mice develop spontaneous tumors of the skin epithelium, no spontaneous reproductive tract malignancies arise, unless the transgenic mice were treated chronically with 17beta-estradiol. These findings suggest that E6 performs critical functions in normal adult cervix and skin, highlighting the need to define E6-controlled transcriptional programs in these tissues.

Methods

We evaluated the expression profile of 14,000 genes in skin or cervix from young K14E6 transgenic mice compared with nontransgenic. To identify differentially expressed genes a linear model was implemented using R and the LIMMA package. Two criteria were used to select the set of relevant genes. First a set of genes with a Log-odds > or = 3 were selected. Then, a hierarch

SUBMITTER: Mendoza-Villanueva D 

PROVIDER: S-EPMC2610035 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature