Type III secretion system 1 genes in Vibrio parahaemolyticus are positively regulated by ExsA and negatively regulated by ExsD.

ABSTRACT: Vibrio parahaemolyticus harbours two distinct type III secretion systems (T3SS1 and T3SS2). A subset of 10 T3SS1 genes are transcribed when V. parahaemolyticus is grown in tissue culture medium [Dulbecco's modified Eagle's medium (DMEM)], while transcription of these genes (except exsD) is minimal upon growth in Luria-Bertani-Salt (LB-S). Transcription of T3SS1 genes and cytotoxicity towards HeLa cells was prevented by deletion of exsA while complementation with exsA restored these traits. Overexpression of ExsA in the wild-type strain, NY-4, activated the transcription of T3SS1 genes when bacteria were grown in LB-S. Thus, ExsA is necessary and sufficient to induce the transcription of T3SS1 genes. Deletion of the exsD permitted the transcription of T3SS1 genes when bacteria were grown in

SUBMITTER: Zhou X 

PROVIDER: S-EPMC2610376 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature