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A large genome center's improvements to the Illumina sequencing system.

ABSTRACT: The Wellcome Trust Sanger Institute is one of the world's largest genome centers, and a substantial amount of our sequencing is performed with 'next-generation' massively parallel sequencing technologies: in June 2008 the quantity of purity-filtered sequence data generated by our Genome Analyzer (Illumina) platforms reached 1 terabase, and our average weekly Illumina production output is currently 64 gigabases. Here we describe a set of improvements we have made to the standard Illumina protocols to make the library preparation more reliable in a high-throughput environment, to reduce bias, tighten insert size distribution and reliably obtain high yields of data.

SUBMITTER: Quail MA

PROVIDER: S-EPMC2610436 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature

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A large genome center's improvements to the Illumina sequencing system.

Quail Michael A MA Kozarewa Iwanka I Smith Frances F Scally Aylwyn A Stephens Philip J PJ Durbin Richard R Swerdlow Harold H Turner Daniel J DJ

Nature methods 20081201 12

The Wellcome Trust Sanger Institute is one of the world's largest genome centers, and a substantial amount of our sequencing is performed with 'next-generation' massively parallel sequencing technologies: in June 2008 the quantity of purity-filtered sequence data generated by our Genome Analyzer (Illumina) platforms reached 1 terabase, and our average weekly Illumina production output is currently 64 gigabases. Here we describe a set of improvements we have made to the standard Illumina protocol ...[more]

PMID: 19034268

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