A quantitative assay for crossover and noncrossover molecular events at individual recombination hotspots in both male and female gametes.

ABSTRACT: Meiotic recombination is a fundamental process in all eukaryotes. Among organisms in which recombination initiates prior to synapsis, recombination preferentially occurs in short 1-to 2-kb regions, known as recombination hotspots. Among mammals, genotyping sperm DNA has provided a means of monitoring recombination events at specific hotspots in male meiosis. To complement these current techniques, we developed an assay for amplifying all copies of a hotspot from the DNA of male and female germ cells, cloning the products into Escherichia coli, and SNP genotyping the resulting colonies using fluorescence technology. This approach examines the molecular details of crossover and noncrossover events of individual meioses directly at active hotspots while retaining the simplicity of using poole

SUBMITTER: Ng SH 

PROVIDER: S-EPMC2610674 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature