Analysis of the Pythium ultimum transcriptome using Sanger and Pyrosequencing approaches.

ABSTRACT:

Background

Pythium species are an agriculturally important genus of plant pathogens, yet are not understood well at the molecular, genetic, or genomic level. They are closely related to other oomycete plant pathogens such as Phytophthora species and are ubiquitous in their geographic distribution and host rage. To gain a better understanding of its gene complement, we generated Expressed Sequence Tags (ESTs) from the transcriptome of Pythium ultimum DAOM BR144 (= ATCC 200006 = CBS 805.95) using two high throughput sequencing methods, Sanger-based chain termination sequencing and pyrosequencing-based sequencing-by-synthesis.

Results

A single half-plate pyrosequencing (454 FLX) run on adapter-ligated cDNA from a normalized cDNA population generated 90,664 reads with an average

SUBMITTER: Cheung F 

PROVIDER: S-EPMC2612028 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature