MtDNA depletion confers specific gene expression profiles in human cells grown in culture and in xenograft.

ABSTRACT:

Background

Interactions between the gene products encoded by the mitochondrial and nuclear genomes play critical roles in eukaryotic cellular function. However, the effects mitochondrial DNA (mtDNA) levels have on the nuclear transcriptome have not been defined under physiological conditions. In order to address this issue, we characterized the gene expression profiles of A549 lung cancer cells and their mtDNA-depleted rho0 counterparts grown in culture and as tumor xenografts in immune-deficient mice.

Results

Cultured A549 rho0 cells were respiration-deficient and showed enhanced levels of transcripts relevant to metal homeostasis, initiation of the epithelial-mesenchymal transition, and glucuronidation pathways. Several well-established HIF-regulated transcripts showed inc

SUBMITTER: Magda D 

PROVIDER: S-EPMC2612029 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature