Differential age-associated regulation of clusterin expression in prostate lobes of brown Norway rats.

ABSTRACT:

Background

Serum androgen concentrations decline with age in male Brown Norway rats and castration induces apoptosis of luminal secretory epithelial cells in the ventral but not in the dorsal and lateral prostate lobes. Clusterin has been described as an androgen-repressed gene and a protein with either anti- or pro-apoptotic actions.

Methods

We measured clusterin mRNA and protein levels, the effects of aging and castration on clusterin protein levels and clusterin immunolocalization within the prostatic ductal network in the prostate lobes of young and aged rats.

Results

Whereas levels of clusterin mRNA and protein expression measured by RT-PCR and Western blot, respectively, were higher in the ventral and lateral lobes of aged (24 months) compared to young (4 months

SUBMITTER: Omwancha J 

PROVIDER: S-EPMC2612093 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature