Genome-wide screen in Francisella novicida for genes required for pulmonary and systemic infection in mice.

ABSTRACT: Francisella tularensis is a gram-negative, highly infectious, aerosolizable facultative intracellular pathogen that causes the potentially life-threatening disease tularemia. To date there is no approved vaccine available, and little is known about the molecular mechanisms important for infection, survival, and dissemination at different times of infection. We report the first whole-genome screen using an inhalation mouse model to monitor infection in the lung and dissemination to the liver and spleen. We queried a comprehensive library of 2,998 sequence-defined transposon insertion mutants in Francisella novicida strain U112 using a microarray-based negative-selection screen. We were able to track the behavior of 1,029 annotated genes, equivalent to a detection rate of 75% and correspondi

SUBMITTER: Kraemer PS 

PROVIDER: S-EPMC2612238 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature