Conservation of the protein composition and electron microscopy structure of Drosophila melanogaster and human spliceosomal complexes.

ABSTRACT: Comprehensive proteomics analyses of spliceosomal complexes are currently limited to those in humans, and thus, it is unclear to what extent the spliceosome's highly complex composition and compositional dynamics are conserved among metazoans. Here we affinity purified Drosophila melanogaster spliceosomal B and C complexes formed in Kc cell nuclear extract. Mass spectrometry revealed that their composition is highly similar to that of human B and C complexes. Nonetheless, a number of Drosophila-specific proteins were identified, suggesting that there may be novel factors contributing specifically to splicing in flies. Protein recruitment and release events during the B-to-C transition were also very similar in both organisms. Electron microscopy of Drosophila B complexes revealed a high de

SUBMITTER: Herold N 

PROVIDER: S-EPMC2612486 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature