The human multidrug resistance protein 4 (MRP4, ABCC4): functional analysis of a highly polymorphic gene.

ABSTRACT: ABCC4 encodes multidrug resistance protein 4 (MRP4), a member of the ATP-binding cassette family of membrane transporters involved in the efflux of endogenous and xenobiotic molecules. The aims of this study were to identify single nucleotide polymorphisms of ABCC4 and to functionally characterize selected nonsynonymous variants. Resequencing was performed in a large ethnically diverse population. Ten nonsynonymous variants were selected for analysis of transport function based on allele frequencies and evolutionary conservation. The reference and variant MRP4 cDNAs were constructed by site-directed mutagenesis and transiently transfected into human embryonic kidney cells (HEK 293T). The function of MRP4 variants was compared by measuring the intracellular accumulation of two antiviral age

SUBMITTER: Abla N 

PROVIDER: S-EPMC2612728 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature