Characterization of fetal and postnatal enteric neuronal cell lines with improvement in intestinal neural function.

ABSTRACT:

Background & aims

The isolation and culture of primary enteric neurons is a difficult process and yields a small number of neurons. We developed fetal and postnatal enteric neuronal cell lines using H-2K(b)-tsA58 transgenic mice (immortomice) that have a temperature-sensitive mutation of the SV40 large tumor antigen gene under the control of an interferon gamma-inducible H-2K(b) promoter element.

Methods

Enteric neuronal precursors were isolated from the intestines of E13-mouse fetuses and second day postnatal mice using magnetic immunoselection with a p75NTR antibody. The cells were maintained at the permissive temperature, 33 degrees C, and interferon-gamma for 24 or 48 hours, and then transferred to 39 degrees C in the presence of glial cell line-derived neurotrophic fact

SUBMITTER: Anitha M 

PROVIDER: S-EPMC2612783 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature