Carboplatin-induced gene expression changes in vitro are prognostic of survival in epithelial ovarian cancer.

ABSTRACT:

Background

We performed a time-course microarray experiment to define the transcriptional response to carboplatin in vitro, and to correlate this with clinical outcome in epithelial ovarian cancer (EOC). RNA was isolated from carboplatin and control-treated 36M2 ovarian cancer cells at several time points, followed by oligonucleotide microarray hybridization. Carboplatin induced changes in gene expression were assessed at the single gene as well as at the pathway level. Clinical validation was performed in publicly available microarray datasets using disease free and overall survival endpoints.

Results

Time-course and pathway analyses identified 317 genes and 40 pathways (designated time-course and pathway signatures) deregulated following carboplatin exposure. Both types of

SUBMITTER: Konstantinopoulos PA 

PROVIDER: S-EPMC2613398 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature