Endothelin receptor B antagonists decrease glioma cell viability independently of their cognate receptor.

ABSTRACT:

Background

Endothelin receptor antagonists inhibit the progression of many cancers, but research into their influence on glioma has been limited.

Methods

We treated glioma cell lines, LN-229 and SW1088, and melanoma cell lines, A375 and WM35, with two endothelin receptor type B (ETRB)-specific antagonists, A-192621 and BQ788, and quantified viable cells by the capacity of their intracellular esterases to convert non-fluorescent calcein AM into green-fluorescent calcein. We assessed cell proliferation by labeling cells with carboxyfluorescein diacetate succinimidyl ester and quantifying the fluorescence by FACS analysis. We also examined the cell cycle status using BrdU/propidium iodide double staining and FACS analysis. We evaluated changes in gene expression by microarray a

SUBMITTER: Montgomery JP 

PROVIDER: S-EPMC2613414 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature