Recombinant expression, purification, and biochemical characterization of chondroitinase ABC II from Proteus vulgaris.

ABSTRACT: Chondroitin lyases (or chondroitinases) are a family of enzymes that depolymerize chondroitin sulfate (CS) and dermatan sulfate (DS) galactosaminoglycans, which have gained prominence as important players in central nervous system biology. Two distinct chondroitinase ABC enzymes, cABCI and cABCII, were identified in Proteus vulgaris. Recently, cABCI was cloned, recombinantly expressed, and extensively characterized structurally and biochemically. This study focuses on recombinant expression, purification, biochemical characterization, and understanding the structure-function relationship of cABCII. The biochemical parameters for optimal activity and kinetic parameters associated with processing of various CS and DS substrates were determined. The profile of products formed by action of cAB

SUBMITTER: Prabhakar V 

PROVIDER: S-EPMC2613618 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature