Overcoming function annotation errors in the Gram-positive pathogen Streptococcus suis by a proteomics-driven approach.

ABSTRACT:

Background

Annotation of protein-coding genes is a key step in sequencing projects. Protein functions are mainly assigned on the basis of the amino acid sequence alone by searching of homologous proteins. However, fully automated annotation processes often lead to wrong prediction of protein functions, and therefore time-intensive manual curation is often essential. Here we describe a fast and reliable way to correct function annotation in sequencing projects, focusing on surface proteomes. We use a proteomics approach, previously proven to be very powerful for identifying new vaccine candidates against Gram-positive pathogens. It consists of shaving the surface of intact cells with two proteases, the specific cleavage-site trypsin and the unspecific proteinase K, followed by LC/MS

SUBMITTER: Rodriguez-Ortega MJ 

PROVIDER: S-EPMC2613929 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature