Egr1 regulates the coordinated expression of numerous EGF receptor target genes as identified by ChIP-on-chip.

ABSTRACT:

Background

UV irradiation activates the epidermal growth factor receptor, induces Egr1 expression and promotes apoptosis in a variety of cell types. We examined the hypothesis that Egr1 regulates genes that mediate this process by use of a chip-on-chip protocol in human tumorigenic prostate M12 cells.

Results

UV irradiation led to significant binding of 288 gene promoters by Egr1. A major functional subgroup consisted of apoptosis related genes. The largest subgroup of 24 genes belongs to the epidermal growth factor receptor-signal transduction pathway. Egr1 promoter binding had a significant impact on gene expression of target genes. Conventional chromatin immunoprecipitation and quantitative real time PCR were used to validate promoter binding and expression changes. Small

SUBMITTER: Arora S 

PROVIDER: S-EPMC2614498 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature